The Human THRSP (Thyroid Hormone Responsive) CLIA Kit is specifically designed for the accurate quantification of Thyroid Hormone Responsive Protein levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it a valuable tool for various research applications.Thyroid Hormone Responsive Protein (THRSP) plays a crucial role in regulating lipid metabolism and energy homeostasis, making it a key player in metabolic disorders such as obesity and diabetes.
By accurately measuring THRSP levels, researchers can gain valuable insights into metabolic pathways and potential therapeutic targets for related conditions.Overall, the Human THRSP CLIA Kit is an essential tool for studying the role of Thyroid Hormone Responsive Protein in metabolic disorders and exploring novel approaches for diagnosis and treatment.
Product Name:
Human THRSP (Thyroid Hormone Responsive) CLIA Kit
SKU:
HUES00798
Target:
Human THRSP (Thyroid Hormone Responsive)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human THRSP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human THRSP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human THRSP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human THRSP. You can calculate the concentration of Human THRSP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
101-117
86-98
Average (%)
95
109
93
1:4
Range (%)
86-96
92-105
84-97
Average (%)
91
98
90
1:8
Range (%)
85-100
88-101
94-105
Average (%)
92
94
100
1:16
Range (%)
100-110
87-101
93-106
Average (%)
105
93
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-96
90
EDTA plasma (n=5)
98-110
104
Cell culture media (n=5)
92-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.72
269.7
860.22
97.91
282.59
789.86
Standard deviation
9.4
22.25
60.39
8.2
27.78
63.5
C V (%)
9.33
8.25
7.02
8.38
9.83
8.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human THRSP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human THRSP in samples. No significant cross-reactivity or interference between Human THRSP and analogues was observed.
