The Human THP (Tamm-Horsfall Glycoprotein) ELISA Kit is specifically designed for the quantitative detection of THP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.THP, also known as uromodulin, is a glycoprotein that is primarily produced by the kidneys and plays a crucial role in the formation of kidney stones. Abnormal levels of THP have been associated with various kidney disorders, making it a valuable biomarker for studying these conditions and potentially developing new diagnostic and therapeutic approaches.
With its advanced technology and user-friendly format, the Human THP ELISA Kit is a valuable tool for researchers and healthcare professionals seeking to investigate the role of THP in kidney diseases and other related disorders. Get your kit today and unlock new insights into the intricate mechanisms underlying these conditions.
Product Name:
Human THP (Tamm–Horsfall Glycoprotein) ELISA Kit
SKU:
HUES02065
Target:
Human THP (Tamm–Horsfall Glycoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human THP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human THP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human THP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human THP. You can calculate the concentration of Human THP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
89-103
85-96
Average (%)
102
95
90
1:4
Range (%)
90-100
84-98
88-99
Average (%)
95
90
93
1:8
Range (%)
87-99
87-101
81-95
Average (%)
92
92
87
1:16
Range (%)
93-106
82-95
87-98
Average (%)
100
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.13
15.74
45.6
4.74
15.97
49.03
Standard deviation
0.35
0.93
2.04
0.24
0.64
2.58
C V (%)
6.82
5.91
4.47
5.06
4.01
5.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human THP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human THP in samples. No significant cross-reactivity or interference between Human THP and analogues was observed.
