The Human THP (Tamm-Horsfall glycoprotein) CLIA Kit is specifically designed for the precise measurement of THP levels in human samples such as serum, plasma, and urine. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research applications.THP, also known as uromodulin, is a key glycoprotein involved in kidney function and plays a crucial role in renal physiology. It serves as a biomarker for kidney diseases, including chronic kidney disease and renal failure, making it an important target for diagnostic and therapeutic research in the field of nephrology.
With its high performance and reliability, the Human THP CLIA Kit is an invaluable tool for researchers studying kidney disorders and seeking to develop novel treatments for these conditions. Get your kit today and enhance your research capabilities in the field of nephrology.
Product Name:
Human THP (Tamm–Horsfall Glycoprotein) CLIA Kit
SKU:
HUES00560
Target:
Human THP (Tamm–Horsfall Glycoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human THP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human THP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human THP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human THP. You can calculate the concentration of Human THP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
87-102
101-116
Average (%)
97
94
107
1:4
Range (%)
90-102
104-117
88-102
Average (%)
95
110
95
1:8
Range (%)
92-106
96-113
98-112
Average (%)
99
104
105
1:16
Range (%)
99-114
92-108
104-118
Average (%)
106
100
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
90
EDTA plasma (n=5)
100-115
107
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.21
7.43
1.05
2.13
7.01
Standard deviation
0.11
0.25
0.67
0.1
0.25
0.51
C V (%)
11.11
11.31
9.02
9.52
11.74
7.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human THP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human THP in samples. No significant cross-reactivity or interference between Human THP and analogues was observed.
