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Human Thioredoxin Binding Protein 2 (TBP2) ELISA Kit

SKU:
HUDL02839
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9H3M7
Sensitivity:
0.066ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
TXNIP,HHCPA78,THIF,VDUP1,Thioredoxin Interacting Protein,Vitamin D3-Upregulated Protein 1,Upregulated By 1,25-Dihydroxyvitamin D-3
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Thioredoxin Binding Protein 2 (TBP2) ELISA Kit

The Human Thioredoxin Binding Protein 2 (TBP2) ELISA Kit is specially designed for the precise and reliable detection of TBP2 levels in human serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring accurate and reproducible results, making it an excellent tool for various research applications.Thioredoxin Binding Protein 2 (TBP2) is a key protein involved in regulating cellular redox balance and oxidative stress response.

It plays a critical role in cell survival, apoptosis, and inflammation, making it a valuable biomarker for studying oxidative stress-related diseases, such as cancer, cardiovascular disorders, and neurodegenerative conditions. The TBP2 ELISA Kit provides researchers with a valuable tool for studying TBP2 levels in different biological samples and exploring its potential role in various disease mechanisms.

Product Name:Human Thioredoxin Binding Protein 2 (TBP2) ELISA Kit
Product Code:HUDL02839
Size:96 Assays
Target:TBP2
Synonyms:TXNIP, HHCPA78, THIF, VDUP1, Thioredoxin Interacting Protein, Vitamin D3-Upregulated Protein 1, Upregulated By 1,25-Dihydroxyvitamin D-3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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