Human TGFA Recombinant Protein (RPPB0967)
- SKU:
- RPPB0967
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P01135
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Human TGFA Recombinant Protein |
| Product Code: | RPPB0967 |
| Size: | 100µg |
| Species: | Human |
| Target: | TGFA |
| Synonyms: | Transforming Growth Factor Alpha, Protransforming Growth Factor Alpha, TGF-Alpha, TGFA. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered solution in 0.1% TFA. |
| Solubility: | It is recommended to reconstitute the lyophilized TGFA in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFA should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA |
| Biological Activity: | The ED50, as measured in a proliferation assay using mouse BALB/c 3T3 cells, is 0.395ng/ml. |
Transforming Growth Factor-Alpha (TGF-alpha) belongs to the EGF family of cytokines. TGFA soluble form is discharged from the membrane by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and has a role in cell-cell adhesion or in the stimulation of adjacent cells. TGFA expression is common in transformed cells. Additionally, TGFA is expressed in normal tissues during embryogenesis and in adult cells/tissues, including the pituitary, keratinocytes, and macrophages.
TGFA Human Recombinant (40-89) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 5.6kDa. The TGFA is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Ligand, receptor tyrosine kinase; Membrane protein, integral; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2p13 Cellular Component: extracellular space; cell surface; basolateral plasma membrane; perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus Molecular Function:protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding Biological Process: response to drug; epidermal growth factor receptor signaling pathway; cell proliferation; positive regulation of epidermal growth factor receptor activity; wound healing; positive regulation of mitosis; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis |
| NCBI Summary: | This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P01135 |
| NCBI GenInfo Identifier: | 135689 |
| NCBI Gene ID: | 7039 |
| NCBI Accession: | P01135.1 |
| UniProt Secondary Accession: | P01135,Q15577, Q53SK7, Q9BS56, Q9UEI3, Q9UKM1, Q9UKM2 Q9UKM3, A8K286, |
| UniProt Related Accession: | P01135 |
| Molecular Weight: | 17,329 Da |
| NCBI Full Name: | Protransforming growth factor alpha |
| NCBI Synonym Full Names: | transforming growth factor, alpha |
| NCBI Official Symbol: | TGFA�� |
| NCBI Official Synonym Symbols: | TFGA�� |
| NCBI Protein Information: | protransforming growth factor alpha; TGF-alpha |
| UniProt Protein Name: | Protransforming growth factor alpha |
| UniProt Synonym Protein Names: | EGF-like TGF; ETGF; TGF type 1 |
| Protein Family: | Transforming growth factor |
| UniProt Gene Name: | TGFA�� |
| UniProt Entry Name: | TGFA_HUMAN |
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