Human TGF- beta3 (Transforming Growth Factor beta3) CLIA Kit
The Human TGF-Beta3 (Transforming Growth Factor Beta3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of TGF-Beta3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it a valuable tool for researchers in various fields.TGF-Beta3 is a key growth factor involved in processes such as cell growth, differentiation, and immune response regulation. It plays a crucial role in various physiological and pathological conditions, including fibrosis, cancer, and immune disorders.
Understanding TGF-Beta3 levels can provide insights into disease mechanisms and potential therapeutic targets.With its high performance and ease of use, the Human TGF-Beta3 CLIA Kit is an essential tool for studying TGF-Beta3 signaling pathways and exploring its potential as a biomarker for various diseases. Get accurate and reliable data with this innovative assay kit from AssayGenie.
Product Name:
Human TGF- beta3 (Transforming Growth Factor beta3) CLIA Kit
SKU:
HUES01211
Target:
Human TGF- beta3 (Transforming Growth Factor beta3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TGF-β3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF-β3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGF-β3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TGF-β3. You can calculate the concentration of Human TGF-β3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
97-111
97-113
Average (%)
91
104
103
1:4
Range (%)
85-96
89-102
103-116
Average (%)
91
94
109
1:8
Range (%)
92-103
98-113
97-113
Average (%)
98
104
104
1:16
Range (%)
86-96
102-116
100-116
Average (%)
91
108
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
107
EDTA plasma (n=5)
98-113
106
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.29
46.6
219.1
27.43
51.24
203.64
Standard deviation
2.41
4.25
15.25
3.12
3.96
13.6
C V (%)
9.53
9.12
6.96
11.37
7.73
6.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TGF-β3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGF-β3 in samples. No significant cross-reactivity or interference between Human TGF-β3 and analogues was observed.
