The Human TG (Thyroglobulin) ELISA Kit is designed for the quantitative detection of Thyroglobulin levels in various biological samples. Thyroglobulin is a key protein involved in thyroid hormone synthesis within the thyroid gland and is essential for normal thyroid function. It serves as the precursor for the production of thyroxine (T4) and triiodothyronine (T3), which play crucial roles in regulating metabolism, growth, and development in the body. Accurate measurement of Thyroglobulin levels is important in assessing thyroid health, monitoring thyroid diseases, and evaluating treatment outcomes.
This ELISA kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under strict quality control standards, the kit delivers robust performance and user-friendly protocols, making it suitable for both research and clinical applications. Trust the Human TG ELISA Kit for precise quantification of this vital biomarker in your studies.
Product Name:
Human TG (Thyroglobulin) ELISA Kit
SKU:
AEES00113
Target:
Human TG (Thyroglobulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TG. You can calculate the concentration of Human TG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-100
92-104
83-93
Average (%)
94
99
90
1:4
Range (%)
90-103
87-99
84-94
Average (%)
96
95
91
1:8
Range (%)
92-102
85-100
87-96
Average (%)
95
92
90
1:16
Range (%)
95-109
90-106
92-100
Average (%)
103
98
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
90-99
93
Cell culture media (n=5)
87-94
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.09
6.05
23.02
2.01
5.36
21.99
Standard deviation
0.11
0.28
1.16
0.12
0.25
1.06
C V (%)
5.26
4.63
5.04
5.97
4.66
4.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TG in samples. No significant cross-reactivity or interference between Human TG and analogues was observed.
