Human TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit
The Human TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit is expertly designed for the precise quantification of Tissue Factor Pathway Inhibitor (TFPI) levels in a variety of biological samples.
TFPI is a crucial anticoagulant protein that regulates blood coagulation and inhibits clot formation by modulating the tissue factor pathway. Its role in hemostasis and thrombosis underscores its significance in maintaining vascular integrity and preventing excessive blood clot formation. Accurate measurement of TFPI is essential for unraveling its contribution to coagulation disorders, cardiovascular diseases, and other pathophysiological conditions. Our TFPI ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and user-friendly operation, making it an excellent choice for cutting-edge research applications. Trust Assay Genie's TFPI ELISA Kit for dependable quantification of this critical anticoagulant protein in your scientific investigations.
Product Name:
Human TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit
SKU:
AEES00166
Target:
Human TFPI (Tissue Factor Pathway Inhibitor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TFPI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TFPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TFPI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TFPI. You can calculate the concentration of Human TFPI in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
84-96
90-105
Average (%)
91
91
96
1:4
Range (%)
82-92
86-98
91-103
Average (%)
87
91
97
1:8
Range (%)
86-102
88-100
89-103
Average (%)
93
93
97
1:16
Range (%)
82-94
81-93
85-100
Average (%)
88
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
84-100
91
Cell culture media (n=5)
90-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.53
107.97
465.09
54.19
103.09
473.23
Standard deviation
3.16
4.65
21.39
3.15
4.16
15.24
C V (%)
6.25
4.31
4.6
5.81
4.04
3.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TFPI concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TFPI in samples. No significant cross-reactivity or interference between Human TFPI and analogues was observed.
