The Human TFF2 (Trefoil Factor 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of TFF2 levels in human biological samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.TFF2 is a key protein involved in mucosal protection and repair, playing a crucial role in maintaining the integrity of the gastrointestinal tract.
Dysregulation of TFF2 levels has been implicated in various gastrointestinal disorders, making it an important biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human TFF2 CLIA Kit offers researchers a valuable tool for investigating the role of TFF2 in health and disease, allowing for the precise quantification of TFF2 levels in human samples.
Product Name:
Human TFF2 (Trefoil Factor 2) CLIA Kit
SKU:
HUES01036
Target:
Human TFF2 (Trefoil Factor 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TFF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TFF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TFF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TFF2. You can calculate the concentration of Human TFF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
87-103
86-98
Average (%)
97
94
91
1:4
Range (%)
91-105
92-108
96-110
Average (%)
97
99
104
1:8
Range (%)
93-107
87-102
101-117
Average (%)
100
94
107
1:16
Range (%)
91-103
92-108
101-114
Average (%)
97
99
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-110
104
EDTA plasma (n=5)
87-101
92
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.78
126.08
347.72
56.55
133.66
378.66
Standard deviation
4.3
10.07
34.53
6.77
13.66
42.41
C V (%)
8.15
7.99
9.93
11.97
10.22
11.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TFF2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TFF2 in samples. No significant cross-reactivity or interference between Human TFF2 and analogues was observed.
