The Transferrin (TF) ELISA Kit is meticulously crafted for the precise quantitative analysis of human TF levels in diverse biological samples. As a critical iron-binding glycoprotein, TF plays a fundamental role in iron transport and homeostasis. Monitoring TF levels is indispensable for investigating iron metabolism, assessing iron deficiency or overload conditions, and understanding various hematological disorders. Accurate measurement of TF provides insights into iron status, transferrin saturation, and overall iron-handling capabilities in the body.
Our TF ELISA Kit ensures exceptional sensitivity and specificity, facilitating reliable and reproducible results in your research and clinical investigations. Manufactured under stringent quality control measures, this kit demonstrates robust performance and user-friendly protocols, making it an optimal choice for a wide range of applications. Count on Assay Genie's TF ELISA Kit for precise and dependable quantification of this pivotal iron regulatory protein in your studies.
Product Name:
Human TF (Transferrin) ELISA Kit
SKU:
AEES00064
Target:
Human TF (Transferrin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 μg/mL
Detection range:
3.13-200 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TF. You can calculate the concentration of Human TF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
92-98
98-109
Average (%)
99
95
103
1:4
Range (%)
89-101
92-106
93-103
Average (%)
93
100
99
1:8
Range (%)
96-107
95-103
88-95
Average (%)
101
98
91
1:16
Range (%)
96-112
85-96
89-102
Average (%)
104
92
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
97
EDTA plasma (n=5)
84-93
90
Cell culture media (n=5)
95-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
8.13
23.58
80.94
8.16
21.11
86.48
Standard deviation
0.48
1.1
3.44
0.47
1.0
4.06
C V (%)
5.9
4.66
4.25
5.76
4.74
4.69
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TF in samples. No significant cross-reactivity or interference between Human TF and analogues was observed.
