Human TERF1 Interacting Nuclear Factor 2 (TINF2) ELISA Kit
The Human TERF1 Interacting Nuclear Factor 2 (TINF2) ELISA Kit is a powerful tool for the quantitative detection of TINF2 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an ideal choice for a variety of research applications.TINF2 is known to be involved in telomere maintenance, a crucial process that protects the ends of chromosomes and plays a key role in cell aging and cancer development.
By measuring TINF2 levels, researchers can better understand the mechanisms of telomere regulation and its implications in aging-related diseases and cancer progression.This ELISA kit provides researchers with a reliable way to study the role of TINF2 in various physiological and pathological processes, offering valuable insights for potential therapeutic interventions. Purchase the Human TERF1 Interacting Nuclear Factor 2 (TINF2) ELISA Kit today and advance your research efforts in telomere biology and related fields.
Product Name:
Human TERF1 Interacting Nuclear Factor 2 (TINF2) ELISA Kit
Product Code:
HUDL02901
Size:
96 Assays
Target:
TINF2
Synonyms:
TIN2, TRF1-interacting nuclear protein 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
