Human Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) ELISA Kit
The Human TERF2IP (Telomeric Repeat Binding Factor 2 Interacting Protein) ELISA Kit is specially designed for the accurate measurement of TERF2IP levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.TERF2IP is a vital protein that interacts with the telomeric repeat binding factor 2 (TERF2) and plays a crucial role in telomere maintenance and stability. Dysregulation of TERF2IP has been linked to genomic instability and various diseases, including cancer and aging-related disorders.
Therefore, measuring TERF2IP levels can provide valuable insights into these conditions and aid in the development of targeted therapeutic interventions.With its high-performance features and broad applicability, the Human TERF2IP ELISA Kit is an essential tool for researchers studying telomere biology, DNA repair mechanisms, and the molecular pathways underlying various diseases. Trust this ELISA kit for accurate and dependable results in your research endeavors.
Product Name:
Human Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) ELISA Kit
Product Code:
HUDL02855
Size:
96 Assays
Target:
TERF2IP
Synonyms:
RAP1, DRIP5, Dopamine receptor-interacting protein 5, Repressor/activator protein 1 homolog
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
