Human Telomerase Associated Protein 1 (TEP1) ELISA Kit
The Human Telomerase-Associated Protein 1 (TEP1) ELISA Kit is specially designed for the precise measurement of TEP1 levels in human biological samples such as serum, plasma, and cell culture supernatants. With excellent sensitivity and specificity, this kit delivers accurate and consistent results, making it an ideal tool for various research applications.TEP1 is a key protein that is associated with telomerase activity, which plays a crucial role in maintaining telomere length and cellular aging. Dysregulation of TEP1 has been linked to various diseases, including cancer and age-related disorders, highlighting its importance as a potential biomarker for studying these conditions and exploring therapeutic interventions.
By utilizing the Human TEP1 ELISA Kit, researchers can gain valuable insights into the role of TEP1 in disease progression and potentially identify novel therapeutic targets for treating associated disorders. Get accurate and reliable data with this advanced ELISA kit from AssayGenie.
Product Name:
Human Telomerase Associated Protein 1 (TEP1) ELISA Kit
Product Code:
HUDL02852
Size:
96 Assays
Target:
TEP1
Synonyms:
TLP1, TP1, TROVE1, VAULT2, p240, TROVE Domain Family Member 1, Telomerase protein component 1, p80 telomerase homolog
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
