Human TECK/CCL25 (Thymus Expressed Chemokine) ELISA Kit
The Human TECK/CCL25 (Thymus Expressed Chemokine) ELISA Kit is a specialized tool meticulously crafted to quantify the levels of Thymus Expressed Chemokine in various human biological samples. TECK/CCL25 is a chemokine known for its crucial role in immune responses and inflammation, particularly in the thymus, where it regulates lymphocyte migration and T cell development. This chemokine's properties and interactions have been implicated in various physiological processes, making its accurate measurement vital in understanding immunological mechanisms.
Our TECK/CCL25 ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research or clinical studies. Produced under strict quality control measures, this kit provides reliable performance and user-friendly protocols, making it well-suited for a range of applications. Count on Assay Genie's TECK/CCL25 ELISA Kit for precise and consistent quantification of this significant biomarker in your research endeavors.
Product Name:
Human TECK/CCL25 (Thymus Expressed Chemokine) ELISA Kit
SKU:
AEES00135
Target:
Human TECK/CCL25 (Thymus Expressed Chemokine)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TECK/CCL25. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TECK/CCL25 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TECK/CCL25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TECK/CCL25. You can calculate the concentration of Human TECK/CCL25 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-103
85-94
92-103
Average (%)
98
91
99
1:4
Range (%)
95-110
97-107
100-106
Average (%)
102
102
103
1:8
Range (%)
87-94
98-108
91-105
Average (%)
90
105
99
1:16
Range (%)
92-104
92-102
89-100
Average (%)
98
96
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-111
104
EDTA plasma (n=5)
93-104
98
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.97
4.44
17.55
1.7
4.23
17.42
Standard deviation
0.1
0.21
0.95
0.09
0.2
0.75
C V (%)
5.08
4.73
5.41
5.29
4.73
4.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TECK/CCL25 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TECK/CCL25 in samples. No significant cross-reactivity or interference between Human TECK/CCL25 and analogues was observed.
