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Human TDP1 Recombinant Protein (RPPB2311)

SKU:
RPPB2311
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NUW8
Research Area:
Enzymes
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Description

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Product Name:Human TDP1 Recombinant Protein
Product Code:RPPB2311
Size:5µg
Species:Human
Target:TDP1
Synonyms:Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, TDP1, FLJ11090, MGC104252.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered clear solution.
Formulation:TDP1 protein solution (0.25mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:ADPMSQEGDY GRWTISSSDE SEEEKPKPDK PSTSSLLCAR QGAANEPRYT CSEAQKAAHK RKISPVKFSN TDSVLPPKRQ KSGSQEDLGW CLSSSDDELQ PEMPQKQAEK VVIKKEKDIS APNDGTAQRT ENHGAPACHR LKEEEDEYET SGEGQDIWDM LDKGNPFQFY LTRVSGVKPK YNSGALHIKD ILSPLFGTLV SSAQFNYCFD VDWLVKQYPP EFRKKPILLV HGDKREAKAH LHAQAKPYEN ISLCQAKLDI AFGTHHTKMM LLLYEEGLRV VIHTSNLIHA DWHQKTQGIW LSPLYPRIAD GTHKSGESPT HFKADLISYL MAYNAPSLKE WIDVIHKHDL SETNVYLIGS TPGRFQGSQK DNWGHFRLKK LLKDHASSMP NAESWPVVGQ FSSVGSLGAD ESKWLCSEFK ESMLTLGKES KTPGKSSVPL YLIYPSVENV RTSLEGYPAG GSLPYSIQTA EKQNWLHSYF HKWSAETSGR SNAMPHIKTY MRPSPDFSKI AWFLVTSANL SKAAWGALEK NGTQLMIRSY ELGVLFLPSA FGLDSFKVKQ KFFAGSQEPM ATFPVPYDLP PELYGSKDRP WIWNIPYVKA PDTHGNMWVP SHHHHHH

TDP1 belongs to the phospholipase D family and contains two PLD phosphodiesterase domains. TDP1 is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. TDP1 may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. Mutations in the TDP1 gene are linked to the disease spinocerebellar ataxia with axonal neuropathy (SCAN1).

TDP1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 617 amino acids (1-608) and having a molecular mass of 69.5kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). TDP1 is fused to 9 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.�

UniProt Protein Function:TDP1: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. Monomer. Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. Belongs to the tyrosyl-DNA phosphodiesterase family.
UniProt Protein Details:

Protein type:Phosphodiesterase; EC 3.1.4.-; DNA repair, damage

Chromosomal Location of Human Ortholog: 14q32.11

Cellular Component: cytoplasm; nucleus

Molecular Function:tyrosyl-DNA phosphodiesterase activity; protein binding; double-stranded DNA binding; exonuclease activity; single-stranded DNA binding

Biological Process: single strand break repair; double-strand break repair; DNA repair

Disease: Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy

NCBI Summary:The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). [provided by RefSeq, Aug 2016]
UniProt Code:Q9NUW8
NCBI GenInfo Identifier:37999797
NCBI Gene ID:55775
NCBI Accession:Q9NUW8.2
UniProt Related Accession:Q9NUW8
Molecular Weight:Predicted Molecular Mass: 20.4kDaAccurate Molecular Mass: 25kDa
NCBI Full Name:Tyrosyl-DNA phosphodiesterase 1
NCBI Synonym Full Names:tyrosyl-DNA phosphodiesterase 1
NCBI Official Symbol:TDP1��
NCBI Protein Information:tyrosyl-DNA phosphodiesterase 1
UniProt Protein Name:Tyrosyl-DNA phosphodiesterase 1
Protein Family:Tyrosyl-DNA phosphodiesterase
UniProt Gene Name:TDP1��
UniProt Entry Name:TYDP1_HUMAN

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