The Human TBG (Thyroxine Binding Globulin) CLIA Kit is specifically designed for the accurate measurement of TBG levels in human serum samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for research purposes.TBG is a vital protein that plays a crucial role in transporting thyroid hormones in the bloodstream. Abnormal levels of TBG can indicate thyroid disorders, making it a valuable biomarker for studying thyroid function and related conditions.
With its easy-to-use format and quick results, the Human TBG CLIA Kit is an essential tool for researchers and clinicians in the field of endocrinology. Order yours today and unlock new insights into thyroid health and hormone regulation.
Product Name:
Human TBG (Thyroxine Binding Globulin) CLIA Kit
SKU:
HUES00844
Target:
Human TBG (Thyroxine Binding Globulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TBG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TBG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TBG. You can calculate the concentration of Human TBG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
101-115
83-96
Average (%)
95
107
90
1:4
Range (%)
100-113
86-96
88-102
Average (%)
107
91
93
1:8
Range (%)
93-106
86-101
93-105
Average (%)
98
93
99
1:16
Range (%)
88-102
86-97
94-109
Average (%)
94
91
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-111
105
EDTA plasma (n=5)
89-99
94
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.19
3.77
0.47
1.26
3.63
Standard deviation
0.05
0.11
0.27
0.06
0.12
0.3
C V (%)
10.87
9.24
7.16
12.77
9.52
8.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TBG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TBG in samples. No significant cross-reactivity or interference between Human TBG and analogues was observed.
