The Human Tau Protein Kinase 1 (TPK1) ELISA Kit is a reliable tool for accurately measuring levels of TPK1 in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and consistent results, making it an excellent choice for various research applications. TPK1 is an important kinase involved in the phosphorylation of tau protein, a key player in Alzheimer's disease and other neurodegenerative disorders. Understanding the role of TPK1 in tau phosphorylation is crucial for unraveling the mechanisms underlying these diseases and developing potential therapeutic interventions.
By using the Human Tau Protein Kinase 1 (TPK1) ELISA Kit, researchers can gain valuable insights into the regulation of tau phosphorylation and its implications in neurodegenerative conditions. This kit is a valuable resource for studying the pathogenesis of Alzheimer's disease and other tau-related disorders, offering a reliable and efficient method for quantifying TPK1 levels in biological samples.
Product Name:
Human Tau-Protein Kinase 1 (tPK1) ELISA Kit
Product Code:
HUDL02971
Size:
96 Assays
Target:
tPK1
Synonyms:
TTBK1, BDTK, TTK, Cdk5/P20, CDK5/P23, GSK, STK31, Brain-derived tau kinase, Tau-Tubulin Kinase 1, Tau-Protein O-Hosphotransferase, Brain Protein Kinase PK40erk
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
