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Human TATA Box Binding Protein Associated Factor 13 (TAF13) ELISA Kit

SKU:
HUDL02822
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q15543
Sensitivity:
0.128ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
TAF2K,TAFII18,Transcription Initiation Factor TFIID Subunit 13,Transcription initiation factor TFIID 18 kDa subunit
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human TATA Box Binding Protein Associated Factor 13 (TAF13) ELISA Kit

The Human TATA Box Binding Protein Associated Factor 13 (TAF13) ELISA Kit is a powerful tool for detecting TAF13 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity for accurate and reproducible results, making it an excellent choice for a variety of research applications.TAF13 is a critical factor that plays a key role in gene transcription by interacting with the TATA box binding protein (TBP) complex.

It is involved in regulating gene expression and has implications in various biological processes. TAF13 is especially relevant in cancer research and other diseases where gene expression is dysregulated.With the Human TAF13 ELISA Kit, researchers can gain valuable insights into the role of TAF13 in disease pathology and potentially identify new therapeutic targets. Order your kit today and take your research to the next level.

Product Name:Human TATA Box Binding Protein Associated Factor 13 (TAF13) ELISA Kit
Product Code:HUDL02822
Size:96 Assays
Target:TAF13
Synonyms:TAF2K, TAFII18, Transcription Initiation Factor TFIID Subunit 13, Transcription initiation factor TFIID 18 kDa subunit
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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