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Human Target Of The Antiproliferative Antibody 1 (TAPA1) ELISA Kit

SKU:
HUDL02828
Product Type:
ELISA Kit
Sensitivity:
0.119ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD81,S5.7,TAPA1,TSPAN28,Tetraspanin 28,Target Of Antiproliferative Antibody 1,26 kDa cell surface protein TAPA-1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Target Of The Antiproliferative Antibody 1 (TAPA1) ELISA Kit

The Human Target of the Antiproliferative Antibody 1 (TAPA1) ELISA Kit is a powerful tool for the precise measurement of TAPA1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.TAPA1, also known as CD81 antigen, is a key protein involved in cell proliferation and immune regulation. Its dysregulation has been linked to various diseases, including cancer and autoimmune disorders.

By detecting TAPA1 levels, researchers can gain valuable insights into disease mechanisms and potentially develop new therapeutic strategies.Overall, the Human Target of the Antiproliferative Antibody 1 (TAPA1) ELISA Kit is an essential tool for studying the role of TAPA1 in health and disease, providing researchers with the reliable data needed to advance knowledge and improve patient outcomes.

Product Name:Human Target Of The Antiproliferative Antibody 1 (TAPA1) ELISA Kit
Product Code:HUDL02828
Size:96 Assays
Target:TAPA1
Synonyms:CD81, S5.7, TAPA1, TSPAN28, Tetraspanin 28, Target Of Antiproliferative Antibody 1, 26 kDa cell surface protein TAPA-1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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