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Human TANK Binding Kinase 1 (TBK1) ELISA Kit

SKU:
HUDL02838
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UHD2
Sensitivity:
0.112ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
NAK,T2K,NF-kappa-B-activating kinase,Serine/threonine-protein kinase TBK1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human TANK Binding Kinase 1 (TBK1) ELISA Kit

The Human Tank-Binding Kinase 1 (TBK1) ELISA Kit is specially designed for the precise quantification of TBK1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.TBK1 is a key enzyme involved in innate immunity and inflammation, playing a critical role in the regulation of immune responses and cell survival.

Dysregulation of TBK1 has been implicated in various diseases such as autoimmune disorders, infectious diseases, and cancer, making it a valuable biomarker for understanding disease mechanisms and developing targeted therapies.With its high-quality components and proven performance, the Human TBK1 ELISA Kit is an essential tool for researchers studying TBK1 signaling pathways and exploring its potential as a therapeutic target in various pathological conditions.

Product Name:Human TANK Binding Kinase 1 (TBK1) ELISA Kit
Product Code:HUDL02838
Size:96 Assays
Target:TBK1
Synonyms:NAK, T2K, NF-kappa-B-activating kinase, Serine/threonine-protein kinase TBK1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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