The Human TAGLN (Transgelin) CLIA Kit is specially designed for the precise measurement of transgelin levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high accuracy and specificity, this kit delivers dependable and consistent results, making it an excellent choice for various research applications.Transgelin, also known as smooth muscle protein 22-α (SM22α), plays a crucial role in regulating smooth muscle contraction and cell migration.
Its dysregulation has been linked to various diseases such as cancer, fibrosis, and cardiovascular disorders, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.With the Human TAGLN CLIA Kit, researchers can efficiently study transgelin levels in human samples, gaining valuable insights into its role in disease progression and potential treatment strategies.
Product Name:
Human TAGLN (Transgelin) CLIA Kit
SKU:
HUES00652
Target:
Human TAGLN (Transgelin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TAGLN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TAGLN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TAGLN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TAGLN. You can calculate the concentration of Human TAGLN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
101-116
99-116
Average (%)
90
108
107
1:4
Range (%)
89-103
98-115
91-101
Average (%)
96
106
96
1:8
Range (%)
90-103
93-111
99-115
Average (%)
95
101
106
1:16
Range (%)
93-106
103-119
98-111
Average (%)
98
110
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
99-111
105
Cell culture media (n=5)
99-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.21
11.25
28.58
4.0
10.73
30.86
Standard deviation
0.52
0.89
2.08
0.46
0.83
2.37
C V (%)
12.35
7.91
7.28
11.5
7.74
7.68
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TAGLN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TAGLN in samples. No significant cross-reactivity or interference between Human TAGLN and analogues was observed.
