Human TAF13 (TATA Box Binding Protein Associated Factor 13) CLIA Kit
The Human TAF13 (TATA Box-binding Protein Associated Factor 13) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of TAF13 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a wide range of research applications.TAF13 is a critical protein that plays a key role in regulating gene expression by interacting with the TATA-binding protein (TBP) to form the TFIID complex. This complex is essential for initiating transcription, making TAF13 an important player in the regulation of gene expression.
Understanding the levels of TAF13 in biological samples can provide valuable insights into various cellular processes and diseases.With its high sensitivity and specificity, the Human TAF13 CLIA Kit is a valuable tool for researchers studying gene expression regulation, transcriptional control, and related pathways. By accurately measuring TAF13 levels, researchers can gain a better understanding of the molecular mechanisms underlying various diseases and potentially identify new therapeutic targets. Advance your research with the Human TAF13 CLIA Kit from AssayGenie.
Product Name:
Human TAF13 (TATA Box Binding Protein Associated Factor 13) CLIA Kit
SKU:
HUES00563
Target:
Human TAF13 (TATA Box Binding Protein Associated Factor 13)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TAF13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TAF13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TAF13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TAF13. You can calculate the concentration of Human TAF13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
97-109
92-106
Average (%)
100
104
98
1:4
Range (%)
97-108
93-109
87-97
Average (%)
102
100
92
1:8
Range (%)
90-105
94-108
93-106
Average (%)
96
100
100
1:16
Range (%)
92-106
88-101
97-113
Average (%)
99
95
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
98
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
215.92
504.85
1976.0
219.49
478.8
2115.1
Standard deviation
26.9
38.22
137.92
20.94
39.26
197.13
C V (%)
12.46
7.57
6.98
9.54
8.2
9.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TAF13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TAF13 in samples. No significant cross-reactivity or interference between Human TAF13 and analogues was observed.
