The Human TACR1 (Tachykinin Receptor 1) CLIA Kit is a reliable and accurate tool for measuring TACR1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.Tachykinin Receptor 1 is a key receptor involved in the regulation of neurogenic inflammation and pain perception. Dysregulation of TACR1 has been linked to various conditions such as chronic pain, inflammatory diseases, and psychiatric disorders, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.
By utilizing the Human TACR1 CLIA Kit, researchers can gain valuable insights into the role of Tachykinin Receptor 1 in disease pathogenesis and explore new avenues for targeted treatment strategies. Trust in the accuracy and reliability of this kit to advance your research and scientific discoveries in the field of TACR1 biology.
Product Name:
Human TACR1 (Tachykinin Receptor 1) CLIA Kit
SKU:
HUES01070
Target:
Human TACR1 (Tachykinin Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TACR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TACR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TACR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TACR1. You can calculate the concentration of Human TACR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
85-99
87-101
Average (%)
104
90
92
1:4
Range (%)
92-106
86-98
102-115
Average (%)
99
92
108
1:8
Range (%)
97-113
98-111
97-112
Average (%)
104
104
103
1:16
Range (%)
87-98
97-111
92-105
Average (%)
92
105
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-114
105
EDTA plasma (n=5)
100-113
106
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.46
577.42
1395.25
222.35
609.64
1509.49
Standard deviation
24.45
63.57
93.48
22.37
46.45
129.97
C V (%)
11.96
11.01
6.7
10.06
7.62
8.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TACR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TACR1 in samples. No significant cross-reactivity or interference between Human TACR1 and analogues was observed.
