The Human Tachykinin Receptor 1 (TACR1) ELISA Kit is specifically designed for the precise detection of TACR1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.TACR1 is a vital receptor involved in the mediation of neurokinin-1 (NK1) signaling, playing a crucial role in regulating pain, inflammation, and stress responses. Dysregulation of TACR1 has been linked to various diseases such as chronic pain conditions, mood disorders, and gastrointestinal disorders, making it a valuable biomarker for understanding these pathologies and exploring potential therapeutic interventions.
With its superior performance and reliability, the Human TACR1 ELISA Kit is an indispensable tool for researchers seeking to unravel the intricate mechanisms of TACR1 signaling and its implications in disease progression and treatment strategies. Get your hands on this cutting-edge kit today and drive your research forward with confidence.
Product Name:
Human Tachykinin Receptor 1 (TACR1) ELISA Kit
Product Code:
HUDL02821
Size:
96 Assays
Target:
TACR1
Synonyms:
NK1R, SPR, NKIR, TAC1R, Neurokinin 1 Receptor, Substance P Receptor
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
