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Human T-Cell, Immune Regulator 1 (TCIRG1)ELISA Kit

SKU:
HUDL02844
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13488
Sensitivity:
8.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
Stv1,Vph1,ATP6N1C,ATP6V0A3,Atp6i,OC-116kDa,OC116,OPTB1,TIRC7,a3,V-Type Proton ATPase 116 kDa Subunit A Isoform 3,Osteoclastic proton pump 116 kDa subunit
Reactivity:
Human
Sample Type:
Serum And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Human T-Cell, Immune Regulator 1 (TCIRG1) ELISA Kit

The Human T-cell Immune Regulator-1 (TCIRG1) ELISA Kit is a reliable and accurate tool for detecting TCIRG1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.TCIRG1 is a key protein involved in regulating T-cell immune responses, playing a crucial role in the immune system's ability to fight off infections and diseases.

Dysregulation of TCIRG1 has been linked to autoimmune disorders, inflammation, and other immune-related conditions, making it a valuable biomarker for studying these diseases and developing potential treatments.With its easy-to-use format and reliable performance, the Human TCIRG1 ELISA Kit is an indispensable tool for researchers seeking to explore the role of TCIRG1 in immune function and disease pathogenesis.

Product Name:Human T-Cell, Immune Regulator 1 (TCIRG1)ELISA Kit
Product Code:HUDL02844
Size:96 Assays
Target:TCIRG1
Synonyms:Stv1, Vph1, ATP6N1C, ATP6V0A3, Atp6i, OC-116kDa, OC116, OPTB1, TIRC7, a3, V-Type Proton ATPase 116 kDa Subunit A Isoform 3, Osteoclastic proton pump 116 kDa subunit
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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