Human Synaptotagmin Like Protein 2 (SYTL2) ELISA Kit
The Human Synaptotagmin-Like Protein 2 (SYTL2) ELISA Kit is specifically designed for the accurate quantification of SYTL2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.SYTL2 is a protein that plays a crucial role in intracellular trafficking and membrane fusion events, making it a key player in cell communication and signaling pathways.
Dysregulation of SYTL2 has been linked to various diseases, including neurological disorders and immune system dysfunction, highlighting its importance as a potential biomarker for disease research and therapeutic development.With its superior performance and ease of use, the Human SYTL2 ELISA Kit is a valuable tool for researchers looking to study the role of SYTL2 in health and disease, ultimately leading to a better understanding of cellular mechanisms and potential treatment options.
Product Name:
Human Synaptotagmin Like Protein 2 (SYTL2) ELISA Kit
Product Code:
HUDL02819
Size:
96 Assays
Target:
SYTL2
Synonyms:
CHR11SYT, SGA72M, SLP2, Exophilin-4, Breast Cancer-Associated Antigen SGA-72M
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
