The Human Syncollin (SYCN) ELISA Kit is a cutting-edge tool specifically designed for the precise measurement of syncollin levels in human samples. This kit offers unparalleled sensitivity and accuracy, guaranteeing consistent and trustworthy results for a variety of research purposes.Syncollin is a key protein involved in the regulation of calcium-dependent exocytosis in insulin-secreting cells. Its expression and function have been linked to various metabolic disorders, making it a valuable biomarker for investigating conditions such as diabetes and obesity.
By studying syncollin levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the Human Syncollin (SYCN) ELISA Kit is an indispensable tool for any researcher interested in studying the role of syncollin in human health and disease. With its high sensitivity and specificity, this kit offers reliable and reproducible results that can drive forward our understanding of syncollin function and its implications for human health.
Product Name:
Human Syncollin (SYCN) ELISA Kit
Product Code:
HUDL02810
Size:
96 Assays
Target:
SYCN
Synonyms:
SYL, INSSA1, Insulin Synthesis Associated 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
