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Human Survivin (Surv) ELISA Kit

SKU:
HUDL02808
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O15392
Sensitivity:
12.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
BIRC5,API4,EPR-1,Baculoviral Inhibitor Of Apoptosis Repeat Containing 5,Apoptosis Inhibitor 4,Survivin Variant 3 Alpha
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Survivin (Surv) ELISA Kit

The Human Survivin ELISA Kit is specifically designed for the precise measurement of survivin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results, making it well-suited for various research purposes.Survivin is a key protein involved in regulating cell division and preventing apoptosis, playing a vital role in cell survival and proliferation.

It is implicated in cancer development, making it a valuable biomarker for cancer research and potential therapeutic interventions.Overall, the Human Survivin ELISA Kit provides researchers with a reliable tool for studying survivin levels in different biological samples, helping advance our understanding of survivin's role in health and disease.

Product Name:Human Survivin (Surv) ELISA Kit
Product Code:HUDL02808
Size:96 Assays
Target:Surv
Synonyms:BIRC5, API4, EPR-1, Baculoviral Inhibitor Of Apoptosis Repeat Containing 5, Apoptosis Inhibitor 4, Survivin Variant 3 Alpha
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)90-10195
EDTA plasma (n=5)91-9995
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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