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Human Surfactant Associated Protein C (SPC) ELISA Kit

SKU:
HUDL02752
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11686
Sensitivity:
0.112ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SFTPC,PSP-C,SFTP2,SP-C,SP5,Pulmonary Surfactant Protein C,Pulmonary surfactant-associated proteolipid SPL(Val)
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Lung Lavage Fluid, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Human Surfactant Associated Protein C (SPC) ELISA Kit

The Human Surfactant Associated Protein C (SPC) ELISA Kit is specifically designed for the accurate measurement of SPC levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results for various research purposes.Surfactant Associated Protein C (SPC) is a critical component involved in the function of pulmonary surfactant, which is essential for maintaining proper lung function and preventing respiratory distress syndrome.

Abnormal levels of SPC have been linked to respiratory conditions such as asthma, chronic obstructive pulmonary disease (COPD), and lung injury, making it a valuable biomarker for studying and developing potential treatments for these disorders.With its reliable performance and broad applications, the Human Surfactant Associated Protein C (SPC) ELISA Kit is an indispensable tool for researchers in the fields of respiratory medicine, pulmonary biology, and drug development.

Product Name:Human Surfactant Associated Protein C (SPC) ELISA Kit
Product Code:HUDL02752
Size:96 Assays
Target:SPC
Synonyms:SFTPC, PSP-C, SFTP2, SP-C, SP5, Pulmonary Surfactant Protein C, Pulmonary surfactant-associated proteolipid SPL(Val)
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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