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Human Surfactant Associated Protein A (SPA) ELISA Kit

SKU:
HUDL02749
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8IWL2
Sensitivity:
0.69ng/mL
Range:
1.875-120ng/mL
ELISA Type:
Sandwich
Synonyms:
PRL,SFTPA,PSAP,PSPA,SFTP,SFTPA1,COLEC4,PSP-A,SFTP1,SFTPA1B,SP-A1,Collectin-4,Alveolar proteinosis protein,35 kDa pulmonary surfactant-associated protein
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Lung Lavage Fluid, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Surfactant Associated Protein A (SPA) ELISA Kit

The Human Surfactant Associated Protein A (SPA) ELISA Kit is a cutting-edge tool for the precise measurement of surfactant associated protein A levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it suitable for a wide range of research applications.Surfactant Associated Protein A (SPA) is a vital component of pulmonary surfactant, playing a crucial role in lung function and respiratory health.

Abnormalities in SPA levels have been associated with various lung diseases such as acute respiratory distress syndrome (ARDS) and cystic fibrosis. Therefore, this kit is an invaluable resource for studying these conditions and potentially discovering new therapeutic approaches.Get your hands on the Human Surfactant Associated Protein A (SPA) ELISA Kit today and unlock the potential for groundbreaking research in respiratory health and lung disease.

Product Name:Human Surfactant Associated Protein A (SPA) ELISA Kit
Product Code:HUDL02749
Size:96 Assays
Target:SPA
Synonyms:PRL, SFTPA, PSAP, PSPA, SFTP, SFTPA1, COLEC4, PSP-A, SFTP1, SFTPA1B, SP-A1, Collectin-4, Alveolar proteinosis protein, 35 kDa pulmonary surfactant-associated protein
Detection Method:Sandwich
Range:1.875-120ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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