Human Suppressors Of Cytokine Signaling 2 (SOCS2) ELISA Kit
The Human Suppressors of Cytokine Signaling 2 (SOCS2) ELISA Kit is a valuable tool for the precise measurement of SOCS2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts superior sensitivity and specificity, guaranteeing accurate and consistent results that are essential for various research purposes.SOCS2 is a key regulator of cytokine signaling pathways, playing a critical role in immune response and inflammation. Dysregulation of SOCS2 has been linked to various diseases including autoimmune disorders, cancer, and metabolic syndromes.
By accurately measuring SOCS2 levels, researchers can gain insights into disease mechanisms and potentially identify new therapeutic targets.The Human SOCS2 ELISA Kit from Assay Genie is a reliable and efficient tool for researchers looking to study the role of SOCS2 in various physiological and pathological processes. Its user-friendly design and robust performance make it an ideal choice for laboratories conducting cutting-edge research in the fields of immunology, oncology, and metabolic diseases.
Product Name:
Human Suppressors Of Cytokine Signaling 2 (SOCS2) ELISA Kit
Product Code:
HUDL02737
Size:
96 Assays
Target:
SOCS2
Synonyms:
CIS2, Cish2, SOCS-2, SSI-2, SSI2, STATI2, Cytokine-inducible SH2 protein 2, STAT-induced STAT inhibitor 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
