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Human Suppressor Of Zeste 12 Homolog (SUZ12) ELISA Kit

SKU:
HUDL02809
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q15022
Sensitivity:
0.113ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CHET9,JJAZ1,Chromatin precipitated E2F target 9 protein,Joined to JAZF1 protein
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Human Suppressor Of Zeste 12 Homolog (SUZ12) ELISA Kit

The Human SUZ12 (Suppressor of Zeste 12 Homolog) ELISA Kit is specifically designed for the quantitative measurement of SUZ12 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.SUZ12 is a key component of the Polycomb Repressive Complex 2 (PRC2), which plays a critical role in gene regulation and chromatin remodeling. Dysregulation of SUZ12 has been implicated in various diseases, including cancer and developmental disorders.

By accurately measuring SUZ12 levels, researchers can gain valuable insights into disease mechanisms and potentially identify novel therapeutic targets.Overall, the Human SUZ12 ELISA Kit provides a valuable tool for studying the role of SUZ12 in disease progression and developing targeted treatments. Order yours today to advance your research efforts in this important area of study.

Product Name:Human Suppressor Of Zeste 12 Homolog (SUZ12) ELISA Kit
Product Code:HUDL02809
Size:96 Assays
Target:SUZ12
Synonyms:CHET9, JJAZ1, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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