Human Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA Kit
The Human Superoxide Dismutase 2 (SOD2) ELISA Kit is a reliable and accurate tool for the detection of SOD2 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, allowing for precise and reproducible results, making it an invaluable resource for a variety of research endeavors.SOD2 is a critical enzyme found in mitochondria that plays a key role in antioxidant defense by neutralizing harmful superoxide radicals. Dysregulation of SOD2 has been implicated in numerous diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, measuring SOD2 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of potential therapeutic interventions.By utilizing the Human SOD2 ELISA Kit, researchers can accurately quantify SOD2 levels in biological samples, enabling a better understanding of its role in disease pathophysiology and potentially identifying novel targets for future treatment strategies.
Product Name:
Human Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA Kit
Product Code:
HUDL02740
Size:
96 Assays
Target:
SOD2
Synonyms:
IPO-B, MNSOD, Mn-SOD
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
