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Human Sulfatase 2 (SULF2) ELISA Kit

SKU:
HUDL02804
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8IWU5
Sensitivity:
0.56ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
HSULF-2,Extracellular sulfatase Sulf-2
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Human Sulfatase 2 (SULF2) ELISA Kit

The Human Sulfatase 2 (SULF2) ELISA Kit is specifically designed for the accurate quantification of SULF2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.SULF2 is an important enzyme that plays a critical role in regulating the sulfation of heparan sulfate proteoglycans, which are involved in various cellular processes such as cell growth, differentiation, and migration.

Dysregulation of SULF2 has been linked to several diseases including cancer, inflammatory disorders, and metabolic diseases, making it a valuable biomarker for understanding these conditions and developing potential therapeutic interventions.With its advanced technology and easy-to-follow protocol, the Human SULF2 ELISA Kit is a valuable tool for researchers looking to study the role of SULF2 in health and disease.

Product Name:Human Sulfatase 2 (SULF2) ELISA Kit
Product Code:HUDL02804
Size:96 Assays
Target:SULF2
Synonyms:HSULF-2, Extracellular sulfatase Sulf-2
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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