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Human Stromal Cell Derived Factor 1 (SDF1) ELISA Kit

SKU:
HUDL02624
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P48061
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CXCL12,PBSF,P-BSF,SCYB12,SDF1-A,SDF1-B,SDF1A,SDF1B,TLSF-A,TLSF-B,TPAR1,Pre-B Cell Growth-Stimulating Factor,Chemokine C-X-C-Motif Ligand 12
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Stromal Cell Derived Factor 1 (SDF1) ELISA Kit

The Human Stromal Cell-Derived Factor-1 (SDF-1) ELISA Kit is a powerful tool for the precise measurement of SDF-1 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it perfect for various research purposes.SDF-1, also known as CXCL12, is a key chemokine involved in recruiting stem cells and immune cells to sites of tissue injury and repair. Its role in cell migration, tissue regeneration, and immune response makes it a valuable target for studying diseases such as cancer, inflammatory disorders, and cardiovascular diseases.

By using the Human Stromal Cell-Derived Factor-1 ELISA Kit, researchers can gain valuable insights into the function of SDF-1 and its role in disease pathology, ultimately leading to the development of innovative therapies and diagnostic tools. Order your kit today and unlock the potential of SDF-1 research in your laboratory.

Product Name:Human Stromal Cell Derived Factor 1 (SDF1) ELISA Kit
Product Code:HUDL02624
Size:96 Assays
Target:SDF1
Synonyms:CXCL12, PBSF, P-BSF, SCYB12, SDF1-A, SDF1-B, SDF1A, SDF1B, TLSF-A, TLSF-B, TPAR1, Pre-B Cell Growth-Stimulating Factor, Chemokine C-X-C-Motif Ligand 12
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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