Human sTREM-1 (soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit
The Assay Genie Human sTREM-1 (soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit is meticulously crafted for the precise quantification of sTREM-1 levels in diverse biological samples.
sTREM-1, a soluble form of the Triggering Receptor Expressed on Myeloid Cells-1, plays a pivotal role in modulating immune responses and inflammatory processes. By accurately measuring sTREM-1 levels, researchers can delve into its significant implications in immune regulation, infectious diseases, and inflammatory disorders, paving the way for a deeper understanding of its biological functions. Our sTREM-1 ELISA Kit is designed to deliver exceptional sensitivity and specificity, ensuring the generation of reliable and reproducible results in your investigative studies. Crafted under stringent quality control measures, this kit provides robust performance and user-friendly protocols, making it an optimal choice for conducting comprehensive and insightful research in the field of sTREM-1 biology. Trust Assay Genie's sTREM-1 ELISA Kit for precise and dependable quantification, empowering your scientific exploration in this critical domain.
Product Name:
Human sTREM-1 (soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit
SKU:
AEES00177
Target:
Human sTREM-1 (soluble Triggering Receptor Expressed on Myeloid Cells-1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sTREM-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sTREM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sTREM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sTREM-1. You can calculate the concentration of Human sTREM-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
93-110
95-109
Average (%)
98
101
100
1:4
Range (%)
90-105
95-110
92-103
Average (%)
96
102
98
1:8
Range (%)
93-107
96-110
92-108
Average (%)
98
103
100
1:16
Range (%)
94-106
98-111
94-110
Average (%)
101
103
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
89-99
94
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.89
15.66
49.46
4.8
15.45
51.27
Standard deviation
0.28
0.79
2.55
0.29
0.7
2.01
C V (%)
5.63
5.03
5.16
6.14
4.55
3.93
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sTREM-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sTREM-1 in samples. No significant cross-reactivity or interference between Human sTREM-1 and analogues was observed.
