The Human STMN1 (Stathmin-1) CLIA Kit is specifically designed to accurately measure levels of Stathmin-1 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a variety of research purposes.Stathmin-1, also known as STMN1, is a key protein involved in regulating microtubule dynamics and cell division. It plays a critical role in various cellular processes, including cell proliferation, migration, and differentiation.
Dysregulation of Stathmin-1 has been linked to several diseases, including cancer, neurodegenerative disorders, and inflammatory conditions, making it a valuable biomarker for understanding these diseases and developing targeted therapies.Overall, the Human STMN1 (Stathmin-1) CLIA Kit is an essential tool for researchers studying the role of Stathmin-1 in disease pathogenesis and exploring its potential as a therapeutic target.
Product Name:
Human STMN1 (Stathmin 1) CLIA Kit
SKU:
HUES01093
Target:
Human STMN1 (Stathmin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human STMN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human STMN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human STMN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human STMN1. You can calculate the concentration of Human STMN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
101-119
101-116
Average (%)
102
108
107
1:4
Range (%)
94-107
100-112
87-101
Average (%)
101
107
94
1:8
Range (%)
97-110
85-98
98-116
Average (%)
104
92
106
1:16
Range (%)
97-113
89-101
90-101
Average (%)
105
94
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
107
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
94-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.3
203.88
912.83
89.42
201.39
904.63
Standard deviation
11.86
19.31
74.94
7.28
17.54
86.48
C V (%)
12.44
9.47
8.21
8.14
8.71
9.56
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human STMN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human STMN1 in samples. No significant cross-reactivity or interference between Human STMN1 and analogues was observed.
