Human sTfR1 (Soluble Transferrin Receptor 1) ELISA Kit
The Human sTfR1 (Soluble Transferrin Receptor 1) ELISA Kit is meticulously crafted to enable the precise quantitative assessment of Soluble Transferrin Receptor 1 levels in diverse human biological samples. Soluble Transferrin Receptor 1 plays a crucial role in the regulation of iron homeostasis by reflecting the cellular iron demand and transport. Accurate measurement of sTfR1 is essential for understanding iron metabolism, hematological disorders, and various iron-related pathologies.
Our ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results in detecting sTfR1 levels. Developed under stringent quality control protocols, this kit exhibits robust performance and ease of use, making it a top choice for both research and clinical applications. Rely on Assay Genie's Human sTfR1 ELISA Kit for dependable quantification of this vital biomarker in your investigative studies.
Product Name:
Human sTfR1 (Soluble Transferrin Receptor 1) ELISA Kit
SKU:
AEES00121
Target:
Human sTfR1 (Soluble Transferrin Receptor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sTfR1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sTfR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sTfR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sTfR1. You can calculate the concentration of Human sTfR1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
85-98
98-108
Average (%)
102
90
104
1:4
Range (%)
97-109
87-99
99-113
Average (%)
105
95
105
1:8
Range (%)
91-101
96-106
89-99
Average (%)
95
99
95
1:16
Range (%)
93-107
89-98
86-95
Average (%)
100
94
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-103
98
EDTA plasma (n=5)
91-103
95
Cell culture media (n=5)
86-93
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
16.79
47.3
172.63
19.28
48.23
198.32
Standard deviation
1
2.31
8.6
1.02
2.32
9.02
C V (%)
5.96
4.88
4.98
5.29
4.81
4.55
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sTfR1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sTfR1 in samples. No significant cross-reactivity or interference between Human sTfR1 and analogues was observed.
