Human Steroid 5 Alpha Reductase 2 (SRD5a2) ELISA Kit
The Human Steroid 5-alpha Reductase 2 (SRD5A2) ELISA Kit is specifically designed for the precise measurement of SRD5A2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.SRD5A2 is an enzyme that plays a crucial role in the conversion of testosterone to dihydrotestosterone (DHT), a potent androgen hormone. Dysregulation of SRD5A2 activity has been linked to several health conditions including prostate cancer, male pattern baldness, and benign prostatic hyperplasia.
Thus, the measurement of SRD5A2 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Human Steroid 5-alpha Reductase 2 (SRD5A2) ELISA Kit is an essential tool for researchers studying steroid metabolism, androgen signaling pathways, and related diseases. Its high performance and reliability make it an invaluable asset in advancing our understanding of SRD5A2 biology and its implications in human health.
Product Name:
Human Steroid 5 Alpha Reductase 2 (SRD5a2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
