The Human sST2 (Soluble ST2) ELISA Kit is specifically developed to quantitatively detect soluble ST2 protein levels in diverse human biological samples. Soluble ST2, a member of the interleukin-1 receptor family, is a crucial biomarker associated with inflammatory responses, allergic reactions, cardiovascular diseases, and other pathological conditions. Accurate measurement of soluble ST2 is imperative for understanding its role in immune regulation and inflammatory processes and for evaluating disease severity and prognosis accurately. Our ELISA kit is meticulously designed to deliver exceptional sensitivity and specificity, ensuring precise and reproducible outcomes in the measurement of soluble ST2 levels.
Manufactured under rigorous quality control standards, this kit offers robust performance, making it an ideal choice for both research studies and clinical applications. Count on the Human sST2 ELISA Kit from Assay Genie for reliable and accurate quantification of this essential biomarker in your scientific inquiries.
Product Name:
Human sST2 (Soluble ST2) ELISA Kit
SKU:
AEES00118
Target:
Human sST2 (Soluble ST2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sST2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sST2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sST2. You can calculate the concentration of Human sST2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-102
91-100
97-109
Average (%)
99
95
102
1:4
Range (%)
94-109
100-110
95-110
Average (%)
102
104
102
1:8
Range (%)
86-96
89-99
91-100
Average (%)
90
94
95
1:16
Range (%)
86-101
95-103
97-106
Average (%)
94
100
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-110
104
EDTA plasma (n=5)
88-97
93
Cell culture media (n=5)
100-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.82
2.46
9.5
0.95
2.17
9.26
Standard deviation
0.05
0.12
0.5
0.05
0.1
0.5
C V (%)
6.1
4.88
5.26
5.26
4.61
5.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sST2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sST2 in samples. No significant cross-reactivity or interference between Human sST2 and analogues was observed.
