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Human Spondin 1 (SPON1) ELISA Kit

SKU:
HUDL02761
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9HCB6
Sensitivity:
0.212ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
VSGP,F-spondin,F-spondin,Extracellular Matrix Protein,Vascular smooth muscle cell growth-promoting factor
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Spondin 1 (SPON1) ELISA Kit

The Human Spondin-1 (Spon1) ELISA Kit is a state-of-the-art assay specifically designed for the precise detection of Spondin-1 levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers unmatched sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications.Spondin-1 is a key protein implicated in various cellular processes, including cell adhesion, migration, and signal transduction.

Its role in developmental processes and organogenesis makes it a valuable target for studying developmental disorders and potential therapeutic interventions.Whether you are investigating cancer progression, neurological disorders, or developmental biology, the Human Spondin-1 ELISA Kit provides a reliable tool for measuring Spondin-1 levels in biological samples, facilitating comprehensive research in diverse fields.

Product Name:Human Spondin 1 (SPON1) ELISA Kit
Product Code:HUDL02761
Size:96 Assays
Target:SPON1
Synonyms:VSGP, F-spondin, F-spondin,Extracellular Matrix Protein, Vascular smooth muscle cell growth-promoting factor
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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