Human Splicing Factor 3B Subunit 3 (SF3B3) ELISA Kit
The Human Splicing Factor 3B Subunit 3 (SF3B3) ELISA Kit is a powerful tool designed for the precise quantification of SF3B3 levels in human samples, including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable asset for a variety of research applications.SF3B3 is a key component of the spliceosome complex, playing a critical role in mRNA splicing and gene expression regulation. Dysregulation of SF3B3 has been implicated in various diseases, including cancer, genetic disorders, and neurological conditions, highlighting its importance as a potential therapeutic target and biomarker for disease monitoring.
By using the Human SF3B3 ELISA Kit, researchers can gain valuable insights into the role of SF3B3 in disease pathology and explore new avenues for drug development and personalized medicine. With its user-friendly format and reliable performance, this kit is an essential addition to any laboratory seeking to advance their understanding of splicing factor biology.
Product Name:
Human Splicing Factor 3B Subunit 3 (SF3B3) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
