Human SPINK1 Recombinant Protein (RPPB2280)
- SKU:
- RPPB2280
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P00995
- Research Area:
- Enzymes
Description
| Product Name: | Human SPINK1 Recombinant Protein |
| Product Code: | RPPB2280 |
| Size: | 20µg |
| Species: | Human |
| Target: | SPINK1 |
| Synonyms: | Serine Peptidase Inhibitor Kazal Type 1, Pancreatic Secretory Trypsin Inhibitor, Tumor-Associated Trypsin Inhibitor, PCTT, TATI, Spink3, Serine Protease Inhibitor Kazal Type 1, TCP, PSTI. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The SPINK1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSDSLGREA KCYNELNGCT KIYDPVCGTD GNTYPNECVL CFENRKRQTS ILIQKSGPC |
Trypsin inhibitor SPINK1 is secreted from pancreatic acinar cells into pancreatic fluid. SPINK1 has a role in inhibition of trypsin-catalyzed premature initiation of zymogens in the pancreas and the pancreatic duct. Alterations in SPINK1 gene are the cause for tropical calcific and pancreatitis hereditary pancreatitis.
SPINK1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 79 amino acids (24-79) and having a molecular mass of 8.6kDa.SPINK1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SPINK1: This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas. Defects in SPINK1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Defects in SPINK1 are the cause of susceptibility to tropical calcific pancreatitis (TCP). TCP is an idiopathic, juvenile, nonalcoholic form of chronic pancreatitis widely prevalent in several tropical countries. It can be associated with fibrocalculous pancreatic diabetes (FCPD) depending on both environmental and genetic factors. TCP differs from alcoholic pancreatitis by a much younger age of onset, pancreatic calcification, a high incidence of insulin dependent but ketosis resistant diabetes mellitus, and an exceptionally high incidence of pancreatic cancer. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 5q32 Cellular Component: extracellular space Molecular Function:serine-type endopeptidase inhibitor activity; protein binding; endopeptidase inhibitor activity Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; regulation of acrosome reaction Disease: Tropical Calcific Pancreatitis; Pancreatitis, Hereditary |
| NCBI Summary: | The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P00995 |
| NCBI GenInfo Identifier: | 124856 |
| NCBI Gene ID: | 6690 |
| NCBI Accession: | P00995.2 |
| UniProt Related Accession: | P00995 |
| Molecular Weight: | 79 |
| NCBI Full Name: | Pancreatic secretory trypsin inhibitor |
| NCBI Synonym Full Names: | serine peptidase inhibitor, Kazal type 1 |
| NCBI Official Symbol: | SPINK1�� |
| NCBI Official Synonym Symbols: | TCP; PCTT; PSTI; TATI; Spink3�� |
| NCBI Protein Information: | pancreatic secretory trypsin inhibitor; tumor-associated trypsin inhibitor; serine protease inhibitor, Kazal type 1 |
| UniProt Protein Name: | Pancreatic secretory trypsin inhibitor |
| UniProt Synonym Protein Names: | Serine protease inhibitor Kazal-type 1; Tumor-associated trypsin inhibitor; TATI |
| Protein Family: | Serine protease inhibitor |
| UniProt Gene Name: | SPINK1�� |
| UniProt Entry Name: | ISK1_HUMAN |
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