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Human Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit

SKU:
HUDL02600
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P21673
Sensitivity:
0.29ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
DC21,KFSD,SAT,SSAT,SSAT-1,Diamine Acetyltransferase 1,Polyamine N-acetyltransferase 1,Putrescine acetyltransferase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit

The Human Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit is a powerful tool for the precise measurement of SAT1 levels in human samples. This kit is specifically designed for the detection of SAT1 in serum, plasma, and cell culture supernatants with high accuracy and sensitivity.SAT1 is an important enzyme involved in polyamine metabolism, playing a critical role in cell growth, proliferation, and apoptosis. Dysregulation of SAT1 has been linked to various diseases, including cancer, metabolic disorders, and neurodegenerative conditions.

As such, studying SAT1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its reliable performance and ease of use, the Human SAT1 ELISA Kit is an essential tool for researchers studying polyamine metabolism and its implications in health and disease. Get accurate and reproducible results with this advanced ELISA kit, and unlock new possibilities in your research endeavors.

Product Name:Human Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit
Product Code:HUDL02600
Size:96 Assays
Target:SAT1
Synonyms:DC21, KFSD, SAT, SSAT, SSAT-1, Diamine Acetyltransferase 1, Polyamine N-acetyltransferase 1, Putrescine acetyltransferase
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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