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Human Spectrin Beta, Non Erythrocytic 4 (SPTbN4) ELISA Kit

SKU:
HUDL02765
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9H254
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
QV,SPNB4,SPTBN3,Beta-IV spectrin,Spectrin, non-erythroid beta chain 3
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Spectrin Beta, Non Erythrocytic 4 (SPTbN4) ELISA Kit

The Human Spectrin Beta Non-Erythrocytic 4 (SPTBN4) ELISA Kit is a specialized assay designed for accurate and precise measurement of SPTBN4 levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring dependable and repeatable results for a variety of research applications.SPTBN4 is an important non-erythrocytic spectrin protein involved in maintaining cell shape, structural support, and protein trafficking within cells.

Dysregulation of SPTBN4 has been linked to neurodegenerative disorders, cancer, and cardiovascular diseases, making it a valuable biomarker for understanding these conditions and developing potential therapeutic interventions.With the Human SPTBN4 ELISA Kit, researchers can accurately quantify SPTBN4 levels in human samples, enabling in-depth investigations into its role in disease pathology and facilitating the development of targeted therapies.

Product Name:Human Spectrin Beta, Non Erythrocytic 4 (SPTbN4) ELISA Kit
Product Code:HUDL02765
Size:96 Assays
Target:SPTbN4
Synonyms:QV, SPNB4, SPTBN3, Beta-IV spectrin, Spectrin, non-erythroid beta chain 3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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