Human SOX9 (Sex Determining Region Y Box Protein 9) CLIA Kit
The Human SOX9 (Sex-Determining Region Y-Box Protein 9) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of SOX9 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for various research applications.SOX9 is a transcription factor that plays a crucial role in the regulation of embryonic development and cell differentiation. Dysregulation of SOX9 has been implicated in various diseases, including skeletal disorders, cancer, and male infertility.
Therefore, measuring SOX9 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its high performance and ease of use, the Human SOX9 CLIA Kit is an essential tool for researchers studying the role of SOX9 in health and disease. Visit our website for more information and to order your kit today: www.assaygenie.com/human-sox9-sex-determining-region-y-box-protein-9-clia-kit-hues00785/
Product Name:
Human SOX9 (Sex Determining Region Y Box Protein 9) CLIA Kit
SKU:
HUES00785
Target:
Human SOX9 (Sex Determining Region Y Box Protein 9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SOX9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SOX9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOX9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SOX9. You can calculate the concentration of Human SOX9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-113
87-100
99-111
Average (%)
107
94
106
1:4
Range (%)
84-97
92-105
89-102
Average (%)
91
98
95
1:8
Range (%)
92-105
101-119
96-112
Average (%)
99
108
104
1:16
Range (%)
100-117
93-106
103-115
Average (%)
108
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
103
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
182.45
482.85
1587.1
172.03
472.42
1434.04
Standard deviation
19.98
48.48
115.38
13.92
53.53
88.19
C V (%)
10.95
10.04
7.27
8.09
11.33
6.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SOX9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SOX9 in samples. No significant cross-reactivity or interference between Human SOX9 and analogues was observed.
