Human Somatostatin receptor type 2 (SSTR2) ELISA Kit (HUEB2330)
- SKU:
- HUEB2330
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P30874
- Range:
- 78-5000 pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- SSTR2, Smstr2, SST2, SSTR2, somatostatin receptor 2, somatostatin receptor type 2, SRIF-1, SS2R, SS-2-R, SS2-R, SRIF-1
- Reactivity:
- Human
Description
Human Somatostatin receptor type 2 (SSTR2) ELISA Kit
The Human Somatostatin Receptor Type 2 (SSTR2) ELISA Kit is specifically designed for the precise measurement of SSTR2 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it an ideal tool for a variety of research purposes.SSTR2 is a critical receptor involved in mediating the effects of somatostatin, a hormone that regulates various physiological functions such as hormone secretion, cell growth, and neurotransmission.
Dysregulation of SSTR2 has been linked to various diseases, including neuroendocrine tumors and pituitary adenomas, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.By using the Human SSTR2 ELISA Kit, researchers can gain valuable insights into the role of SSTR2 in disease pathology and treatment, ultimately advancing our understanding of these complex conditions and paving the way for novel therapeutic approaches.
| Product Name: | Human Somatostatin receptor type 2 (SSTR2) ELISA Kit |
| SKU: | HUEB2330 |
| Size: | 96T |
| Target: | Human Somatostatin receptor type 2 (SSTR2) |
| Synonyms: | SRIF-1, SS-2-R |
| Assay Type: | Sandwich |
| Detection Method: | ELISA |
| Reactivity: | Human |
| Detection Range: | 78-5000pg/mL |
| Sensitivity: | 33pg/mL |
| Intra CV: | 5.6% | ||||||||||||||||||||
| Inter CV: | 9.6% | ||||||||||||||||||||
| Linearity: |
| ||||||||||||||||||||
| Recovery: |
| ||||||||||||||||||||
| Function: | Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. Inhibits calcium entry by suppressing voltage-dependent calcium channels. Acts as the functionally dominant somatostatin receptor in pancreatic alpha- and beta-cells where it mediates the inhibitory effect of somatostatin-14 on hormone secretion. Inhibits cell growth through enhancement of MAPK1 and MAPK2 phosphorylation and subsequent up-regulation of CDKN1B. Stimulates neuronal migration and axon outgrowth and may participate in neuron development and maturation during brain development. Mediates negative regulation of insulin receptor signaling through PTPN6. Inactivates SSTR3 receptor function following heterodimerization. |
| Uniprot: | P30874 |
| Sample Type: | Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids |
| Specificity: | Natural and recombinant human Somatostatin receptor type 2 |
| Sub Unit: | Homodimer and heterodimer with SSTR3 and SSTR5. Heterodimerization with SSTR3 inactivates SSTR3 receptor function. Heterodimerization with SSTR5 is enhanced by agonist stimulation of SSTR2 and increases SSTR2 cell growth inhibition activity. Following agonist stimulation, homodimers dissociate into monomers which is required for receptor internalization. Interacts with beta-arrestin; this interaction is necessary for receptor internalization and is destabilized by heterodimerization with SSTR5 which results in increased recycling of SSTR2 to the cell surface. Interacts (via C-terminus) with SHANK1 (via PDZ domain). |
| Subcellular Location: | Cell membrane Multi-pass membrane protein Cytoplasm Located mainly at the cell surface under basal conditions. Agonist stimulation results in internalization to the cytoplasm. |
| Storage: | Please see kit components below for exact storage details |
| Note: | For research use only |
| UniProt Protein Function: | SSTR2: Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels. The C-terminus interacts with SHANK1 PDZ domain. Cerebrum and kidney. In lesser amounts in jejunum, colon and liver. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 17q24 Cellular Component: neuron projection; integral to plasma membrane; cytoplasm; plasma membrane Molecular Function:neuropeptide binding; protein binding; somatostatin receptor activity; PDZ domain binding Biological Process: peristalsis; regulation of muscle contraction; response to starvation; synaptic transmission; negative regulation of cell proliferation; G-protein signaling, coupled to cyclic nucleotide second messenger; cell-cell signaling; neuropeptide signaling pathway; forebrain development; digestion; cerebellum development; spermatogenesis; G-protein signaling, adenylate cyclase inhibiting pathway; response to nutrient |
| NCBI Summary: | Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SSTR2 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in cerebrum and kidney. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30874 |
| NCBI GenInfo Identifier: | 401126 |
| NCBI Gene ID: | 6752 |
| NCBI Accession: | P30874.1 |
| UniProt Secondary Accession: | P30874,Q4VBP0, Q96GE0, Q96TF2, Q9BWH1, A8K3Y0, B2R9P7 |
| UniProt Related Accession: | P30874 |
| Molecular Weight: | 369 |
| NCBI Full Name: | Somatostatin receptor type 2 |
| NCBI Synonym Full Names: | somatostatin receptor 2 |
| NCBI Official Symbol: | SSTR2 |
| NCBI Protein Information: | somatostatin receptor type 2; SS2R; SRIF-1 |
| UniProt Protein Name: | Somatostatin receptor type 2 |
| UniProt Synonym Protein Names: | SRIF-1 |
| UniProt Gene Name: | SSTR2 |
| UniProt Entry Name: | SSR2_HUMAN |
| Component | Quantity (96 Assays) | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | -20°C |
| Lyophilized Standard | 2 | -20°C |
| Sample Diluent | 20ml | -20°C |
| Assay Diluent A | 10mL | -20°C |
| Assay Diluent B | 10mL | -20°C |
| Detection Reagent A | 120µL | -20°C |
| Detection Reagent B | 120µL | -20°C |
| Wash Buffer | 30mL | 4°C |
| Substrate | 10mL | 4°C |
| Stop Solution | 10mL | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.
| Step | |
| 1. | Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C. |
| 2. | Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform. |
| 3. | Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper. |
| 4. | Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C. |
| 5. | Repeat the wash process for five times as conducted in step 3. |
| 6. | Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction. |
| 7. | Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing. |
| 8. | Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters. |
| 9. | After experiment, store all reagents according to the specified storage temperature respectively until their expiry. |
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
| Sample Type | Protocol |
| Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
Related Products
Human Somatostatin receptor type 2 / SSTR2 ELISA Kit
Mouse Sstr2 (Somatostatin receptor type 2)ELISA Kit
Rat Somatostatin receptor type 2 (Sstr2) ELISA Kit (RTEB1477)
Porcine Somatostatin receptor type 2 (SSTR2) ELISA Kit (PREB0577)
Rat SSTR2 (Somatostatin receptor type 2) ELISA Kit (RTFI01406)
Mouse Somatostatin receptor type 2 (Sstr2) ELISA Kit (MOEB2079)
Bovine Somatostatin receptor type 2 (SSTR2) ELISA Kit (BOEB1038)
Dog Somatostatin receptor type 2 (SSTR2) ELISA Kit (CNEB0347)
