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Human Solute Carrier Family 39, Member 6 (SLC39A6) ELISA Kit

SKU:
HUDL02709
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13433
Sensitivity:
0.114ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
LIV-1,Zinc Transporter Protein 6,Estrogen-regulated protein LIV-1,Zrt- and Irt-like protein 6,Zinc transporter ZIP6
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Solute Carrier Family 39, Member 6 (SLC39A6) ELISA Kit

The Human SLC39A6 ELISA Kit is specifically designed for the precise measurement of solute carrier family 39 member 6 (SLC39A6) levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.SLC39A6, also known as ZIP6, is a critical transporter protein responsible for the regulation of cellular zinc homeostasis. Dysregulation of SLC39A6 has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders.

Thus, measuring SLC39A6 levels can provide valuable insights into the pathogenesis of these conditions and may aid in the development of targeted therapeutics.Overall, the Human SLC39A6 ELISA Kit is a valuable tool for researchers looking to study the role of SLC39A6 in health and disease, offering accurate and reliable results for their investigations.

Product Name:Human Solute Carrier Family 39, Member 6 (SLC39A6) ELISA Kit
Product Code:HUDL02709
Size:96 Assays
Target:SLC39A6
Synonyms:LIV-1, Zinc Transporter Protein 6, Estrogen-regulated protein LIV-1, Zrt- and Irt-like protein 6, Zinc transporter ZIP6
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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