Human Solute Carrier Family 3, Member 2 (SLC3A2) ELISA Kit
SKU:
HUDL02710
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08195
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC3A2,CD98HC,4F2,4F2HC,4T2HC,MDU1,NACAE,Activators Of Dibasic And Neutral Amino Acid Transport,Antigen Identified By Monoclonal Antibodies 4F2,CD98 Heavy Chain
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human Solute Carrier Family 3, Member 2 (SLC3A2) ELISA Kit
The Human SLC3A2 ELISA Kit is specifically designed for the precise measurement of solute carrier family 3 member 2 (SLC3A2) levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.SLC3A2 is a key membrane protein involved in the transport of amino acids across cell membranes, playing a critical role in nutrient uptake and cellular metabolism. Dysregulation of SLC3A2 has been linked to various diseases such as cancer, metabolic disorders, and neurological conditions, making it a valuable biomarker for understanding these pathologies and developing therapeutic interventions.
By utilizing the Human SLC3A2 ELISA Kit, researchers can gain valuable insights into the role of SLC3A2 in disease progression and explore its potential as a target for novel treatment strategies. This kit is easy to use, reliable, and provides accurate results, making it a valuable tool for advancing scientific research in the field of SLC3A2 biology.
Product Name:
Human Solute Carrier Family 3, Member 2 (SLC3A2) ELISA Kit
Product Code:
HUDL02710
Size:
96 Assays
Target:
SLC3A2
Synonyms:
SLC3A2, CD98HC, 4F2, 4F2HC, 4T2HC, MDU1, NACAE, Activators Of Dibasic And Neutral Amino Acid Transport, Antigen Identified By Monoclonal Antibodies 4F2, CD98 Heavy Chain
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
