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Human Solute Carrier Family 26,Member 4 (SLC26A4) ELISA Kit

SKU:
HUDL02700
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O43511
Sensitivity:
0.121ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
Pendrin,DFNB4,PDS,Sodium-Independent Chloride/Iodide Transporter
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Solute Carrier Family 26,Member 4 (SLC26A4) ELISA Kit

The Human SLC26A4 (Solute Carrier Family 26 Member 4) ELISA Kit is a powerful tool for accurately measuring SLC26A4 levels in human samples including serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit delivers precise and consistent results, making it an excellent choice for various research applications.SLC26A4 is a critical member of the solute carrier family, playing a crucial role in transporting ions across cell membranes.

Dysregulation of SLC26A4 has been linked to various health conditions such as hearing loss, thyroid disorders, and kidney diseases. Thus, this ELISA kit serves as a valuable tool for studying these conditions and exploring potential therapeutic interventions.Overall, the Human SLC26A4 ELISA Kit offers researchers a reliable and efficient method for quantifying SLC26A4 levels, providing valuable insights into its role in health and disease.

Product Name:Human Solute Carrier Family 26,Member 4 (SLC26A4) ELISA Kit
Product Code:HUDL02700
Size:96 Assays
Target:SLC26A4
Synonyms:Pendrin, DFNB4, PDS, Sodium-Independent Chloride/Iodide Transporter
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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